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HM2 and HM2 TUVO High-Mass Systems
Interaction Analysis Kits and Reagents
Complex Tracker Software


HM2 and HM2 TUVO High-Mass Systems

MALDI-TOF MS·Î º»¿¬ÀÇ ´Ü¹éÁú º¹ÇÕü(intact protein complexes)¸¦ ºÐ¼®Çϱâ À§Çؼ­´Â Ưº°È÷ °í¾ÈµÈ
°íÁú·® °ËÃâ½Ã½ºÅÛÀÌ ÇÊ¿äÇÕ´Ï´Ù. CovalXÀÇ µ¶Æ¯ÇÑ conversion dynode ±â¼úÀ» Åä´ë·Î ÇÑ Â÷¼¼´ë °íÁú·®
½Ã½ºÅÛÀ» ¼Ò°³ÇÕ´Ï´Ù. HM2¿Í HM2 TUVO High-Mass SystemÀº nM °¨µµ·Î ±× µ¿¾È ±ÙÁ¢Çϱ⠾î·Á¿ü´ø ÃÖ´ë 1.2MDa±îÁöÀÇ °Å´ëºÐÀÚÀÌ¿ÂÀ» °ËÃâÇØ ³À´Ï´Ù. HM2 High-Mass SystemÀº °Å´ë°íºÐÀÚ Áú·®ÃøÁ¤ÀÇ »õ·Î¿î
±âÁØÀÔ´Ï´Ù. HM2 TUVO´Â HM2ÀÇ ±â´É°ú ´õºÒ¾î ¾ç±Ø/À½±Ø ¸ðµç ¸ðµå¿¡¼­ ÀÌ¿Â ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù.

 

HM2 ¿Í HM2 TUVO High-Mass SystemÀº ¸ðµç Ç¥ÁØ MALDI TOF MS¿¡¼­ ÀÌ¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù :
 
¡¤ Applied Biosystems: 5800, 4800, Voyager series (DE Pro, DE STR).
¡¤ Bruker: biflex, reflex, autoflex and ultraflex series.
¡¤ Shimadzu Kratos: Axima series.
 
CovalX HM2 High-Mass SystemÀº MALDI-TOF MSÀÇ ÀÌÀü ±â´É¿¡ ¾Æ¹«·± º¯È­¸¦ ÁÖÁö ¾Ê½À´Ï´Ù. °³Á¶ ÈÄ¿¡µµ,
¼±Çü¸ðµåÀÇ Ç¥ÁØ °ËÃâ±â (linear mode detector)¸¦ Á¦°ÅÇÏÁö ¾Ê°í ±×´ë·Î »ç¿ëÇÕ´Ï´Ù. CovalXÀÇ ¾ÆÀÌÆÌ ÅÍÄ¡
ÄÁÆ®·Ñ·¯ ¹öÆ°À» °£´ÜÈ÷ ´­·¯ ¸î ÃÊ ¸¸¿¡ Ç¥ÁØ °ËÃâ°ú °íÁú·® °ËÃâ Áß¿¡¼­ Çϳª¸¦ °£´ÜÈ÷ ¼±ÅÃÇÒ ¼ö ÀÖ½À´Ï´Ù.
 
CovalX HM2 High-Mass SystemÀ¸·Î ´ÙÀ½°ú °°Àº ÀÀ¿ëºÐ¾ß¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
 
¡¤ º»¿¬ÀÇ ´Ü¹éÁú º¹ÇÕü ºÐ¼® (intact protein complex analysis)
¡¤ Ç×ü Ư¼ºÈ­ (antibody characterization)
¡¤ Ä¡·á¿ë ´Ü¹éÁúÀÇ ÀÀÁý (therapeutic proteins aggregates)
 
HM2 High-Mass SystemÀº °íºÐÀÚ Áú·® °Å´ëºÐÀÚ¿Í º¹ÀâÇÑ ´Ü¹éÁú È¥ÇÕ¹°¿¡ ´ëÇؼ­ ¶Ù¾î³­ °¨µµ¸¦ °¡Áö°í
ÀÖ½À´Ï´Ù.
 
HM2 ¿Í HM2 TUVO¿ë CovalX ¾ÆÀÌÆÌ ÅÍÄ¡
ÄÁÆ®·Ñ·¯


HM2 ¿Í HM2 TUVO´Â CovalXÀÇ ¾ÆÀÌÆÌ ÅÍÄ¡ ÀÀ¿ë
ÇÁ·Î±×·¥À¸·Î ¹«¼± Á¦¾îµË´Ï´Ù. CovalX ¾ÆÀÌÆÌ ÅÍÄ¡ ÄÁÆ®·Ñ·¯ÀÇ ¹öÆ°À» °£´ÜÈ÷ ´©¸£¸é, °íÁú·® °ËÃâ°ú
Ç¥ÁØ °ËÃ⠶Ǵ ¾çÀ̿°ú À½ÀÌ¿Â °ËÃâ¸ðµå (TUVO)·Î
ÀüȯµË´Ï´Ù.
  High-Mass MALDI-TOF mass spectra of the
immunoglobulin IgM (300 nM).

The mono-charged pseudo-molecular ions
of IgM are detected with m=973.26 kDa.
 
HM2 TUVO High-Mass System retrofit on ABI MALDI TOF/TOF 5800. More information...
 
 
 
 

 

HM2 and HM2 TUVO High-Mass Systems
Interaction Analysis Kits and Reagents
Complex Tracker Software

 

Interaction Analysis Kits and Reagents

MALDI-TOF Áú·®ºÐ¼®±â¸¦ ÀÌ¿ëÇÏ¿© ´Ü¹éÁú º¹ÇÕü¸¦ ºÐ¼®ÇÏ´Â µ¿¾È ´Ü¹éÁú º¹ÇÕü°¡ ºØ±«µÇ´Â °æÇâ ¶§¹®¿¡
´ëºÎºÐ ³ëµ¿Áý¾àÀûÀ̾î¾ß ÇÕ´Ï´Ù. ÀÌ·¯ÇÑ ºÒÆíÀ» ÇØ°áÇÏ°íÀÚ CovalX´Â High-Mass MALDI-TOF Áú·®ºÐ¼®±â¸¦
ÀÌ¿ëÇÑ °£ÆíÇÏ°í ½Å¼ÓÇÑ ´Ü¹éÁú º¹ÇÕü ºÐ¼®¹æ¹ýÀ» °³¹ßÇÏ¿´½À´Ï´Ù. ºÐ¼® ù ´Ü°è´Â Àü¿ë cross-linking ½Ã¾à°ú
ÇÁ·ÎÅäÄÝÀ» »ç¿ëÇÏ¿© °ü½É ÀÖ´Â ºñ°øÀ¯ º¹ÇÕü¸¦ ¾ÈÁ¤È­ÇÏ´Â °ÍÀÔ´Ï´Ù.
 
 
High-Mass MALDI ToF mass spectra of the protein complex Thymidine Kinase (TK).
A. TK (1ul) analyzed without cross-linking. Only the monomer is detected. B. TK
analyzed after cross-linking with disuccimidyl suberate (0.1 mg/ml, 30 minutes
incubation time). TK dimer is detected with m=87.8 kDa. C. Same analysis with
CovalX K100 analysis reagent (0.1 mg/ml, 30 minutes incubation time).
 

High-Mass MALDI TOF Áú·®ºÐ¼®±â·Î º»¿¬ÀÇ ´Ü¹éÁú º¹ÇÕü(intact protein complex)¸¦ ºÐ¼®Çϱâ
À§Çؼ­´Â, ƯÈ÷ °íÈ¿À² cross-linking ½Ã¾àÀ» ÀÌ¿ëÇÑ º¹ÇÕüÀÇ ¾ÈÁ¤È­°¡ Áß¿äÇÕ´Ï´Ù. CovalX´Â
High-Mass MALDI ºÐ¼®À» À§ÇÑ ºñ°øÀ¯ ´Ü¹éÁú º¹ÇÕü Áغñ Àü¿ë ½Ã¾à°ú buffer¸¦ °³¹ßÇÏ¿´½À´Ï´Ù.
cross-linking È¿À²À» ³ôÀ̱â À§ÇØ, CovalX ½Ã¾àÀº ÃÖ°íÀÇ È¿À²·Î ƯÁ¤ ´Ü¹éÁú º¹ÇÕü¸¦ °øÀ¯ÇÒ ¼ö
ÀÖµµ·Ï ±æÀÌ°¡ ´Ù¸¥ spacer¸¦ °¡Áø cross-linkers cocktailÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. CovalX cross-linking
½Ã¾àÀÇ Æ¯À̼ºÀ¸·Î ¿À¿°µÇ¾ú°Å³ª Á¤Á¦µÇÁö ¾ÊÀº »ùÇÿ¡¼­µµ ´Ü¹éÁú º¹ÇÕü¸¦ °øÀ¯ ¾ÈÁ¤È­ ÇÒ ¼ö ÀÖ½À´Ï´Ù.
 

HM2 and HM2 TUVO High-Mass Systems
Interaction Analysis Kits and Reagents
Complex Tracker Software


Complex Tracker Software

º¹ÀâÇÑ ´Ü¹éÁú È¥ÇÕ¹° Áß¿¡¼­ ´Ü¹éÁú »óÈ£ÀÛ¿ëÀ» ºÐ¼®ÇÒ ¶§, High-Mass MALDI-TOF MS·Î ¾òÀº º¹ÀâÇÑ
µ¥ÀÌÅ͸¦ ó¸®ÇÒ ¼ÒÇÁÆ®¿þ¾î°¡ ÇÊ¿äÇÕ´Ï´Ù. ¿©·¯ºÐÀÇ Ãëµæ ¼ÒÇÁÆ®¿þ¾î (acquisition software)¸¦ ¹Ù²Ü ÇÊ¿ä
¾øÀÌ, CovalXÀÇ complex tracker·Î ¸ðµç Ç¥ÁØ MALDI-TOF MS ÆÄÀÏÀ» ºÒ·¯¿É´Ï´Ù. ºÒ·¯¿Â ÈÄ, µ¥ÀÌÅ͸¦
ºñ°øÀ¯ º¹ÇÕüÀÇ Á¸Àç¿©ºÎ¸¦ Åë°èÀûÀ¸·Î Æò°¡Çϱâ À§ÇÑ ÇÁ·ÎÁ§Æ®·Î °ü¸®ÇÕ´Ï´Ù.
 
 
ÇÁ·ÎÁ§Æ® ¸Å´ÏÀú·Î, °°Àº È­¸é¿¡¼­ ¿©·¯ ½ÖÀÇ (control/cross-link) ½ºÆåÆ®·³À» ºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù.
º¹ÀâÇÑ °ËÃâ Æò°¡µµ±¸¸¦ ÀÌ¿ëÇÏ¿© ºñ°øÀ¯ º¹ÇÕüÀÇ Á¸Àç¿©ºÎ¸¦ Åë°èÀûÀ¸·Î °è»êÇÕ´Ï´Ù.