13C Stable Isotope Labeled Glycan Standards
CarboQuant standards는 질량분석기를 이용한 glycan 절대 정량용 internal standard로 사용하기 위한 합성 C13 labeled N-glycan입니다. Single standards 또는 정량 소프트웨어와 함께 glycan 분석에 필요한 모든 시약 및 소모품을 포함한 키트 형태로 제공됩니다. 완벽하게 합성하여 MS와 NMR로 특성분석한 모든 N-acetylglucosamine은 최대 10 Da 질량 차이로 13C stable isotope로 labeled 되어 peak가 중복되지 않습니다.
CarboQuant standards
- >95% 순도
- MS와 NMR로 특성분석
- 최대 10 Da mass gain. No peak overlap
- 모든 N-acetylglucosamine에 13C stable isotope표지
- Quantified internal standards (qNMR )
- Broad coverage of N-glycan structures
Applications
- Internal glycan standards를 사용한 glycan 분석 workflow 개선
- Internal glycan standards를 사용한 분석법의 견고성 향상
- Biofluids에 포함된 개별 glycans의 정량화
- MALDI-TOF MS를 이용한 매우 빠른 샘플 준비 및 데이터 수집을 통해 바이오마커 연구 및 바이오의약품 연구에서 정량적 glycan profiles 생성
Glycan Standard 13C Paucimannose Man3
- 2 x 13C per GlcNAc
- +4 Da mass gain
- Molecular Formula : C3013C4H58N2O26
- Monoisotopic mass : 914.34098
| P/N | Volume |
| ILGM3-600pm | 3x200 pmol |
| ILGM3-2000pm | 10x200 pmol |
Glycan Standard 13C Paucimannose Man5
- 2 x 13C per GlcNAc
- +4 Da mass gain
- Molecular Formula : C4213C4H78N2O36
- Monoisotopic mass : 1238.44663
| P/N | Volume |
| ILGM5-600pm | 3x200 pmol |
| ILGM5-2000pm | 10x200 pmol |
Glycan Standard 13C truncated MGn(6)
- 2 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C3613C6H71N3O31
- Monoisotopic mass : 1119.42084
| P/N | Volume |
| ILMGn(6)-600pm | 3x200 pmol |
| ILMGn(6)-2000pm | 10x200 pmol |
Glycan Standard 13C truncated MGn(3)
- 2 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C4213C6H81N3O35
- Monoisotopic mass : 1265.48519
| P/N | Volume |
| ILMGn(3)F-600pm | 3x200 pmol |
| ILMGn(3)F-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G0
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C4213C8H84N4O36
- Monoisotopic mass : 1324.51337
| P/N | Volume |
| ILGG0-600pm | 3x200 pmol |
| ILGG0-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G0F
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C4813C8H94N4O40
- Monoisotopic mass : 1470.57127
| P/N | Volume |
| ILGG0F-600pm | 3x200 pmol |
| ILGG0F-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G1(3)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C4813C8H94N4O41
- Monoisotopic mass : 1486.56619
| P/N | Volume |
| ILGG1(3)-600pm | 3x200 pmol |
| ILGG1(3)-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G1(6)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C4813C8H94N4O41
- Monoisotopic mass : 1486.56619
| P/N | Volume |
| ILGG1(6)-600pm | 3x200 pmol |
| ILGG1(6)-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G1(6)F
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C5413C8H104N4O45
- Monoisotopic mass : 1486.56619
| P/N | Volume |
| ILGG1(6)F-600pm | 3x200 pmol |
| ILGG1(6)F-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G2
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C5413C8H104N4O46
- Monoisotopic mass : 1648.61901
| P/N | Volume |
| ILGG2-600pm | 3x200 pmol |
| ILGG2-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G2F
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C6013C8H114N4O50
- Monoisotopic mass : 1796.91848
| P/N | Volume |
| ILGG2F-600pm | 3x200 pmol |
| ILGG2F-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary A1(3)(Sial α-2,3)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C6513C8H121N5O54
- Monoisotopic mass : 1777.6616
| P/N | Volume |
| ILGA13S23-600pm | 3x200 pmol |
| ILGA13S23-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary A1(6)F(Sial α-2,3)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C6513C8H121N5O53
- Monoisotopic mass : 1923.71951
| P/N | Volume |
| ILGA16FS23-600pm | 3x200 pmol |
| ILGA16FS23-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G2A1(3)(Sial α-2,3)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C6513C8H121N5O54
- Monoisotopic mass : 1936.71443
| P/N | Volume |
| ILGG2A13S23-600pm | 3x200 pmol |
| ILGG2A13S23-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G2A1(6)F(Sial α-2,3)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C7113C8H131N5O58
- Monoisotopic mass : 2085.77234
| P/N | Volume |
| ILGG2A16FS23-600pm | 3x200 pmol |
| ILGG2A16FS23-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary A2 2x(Sial α-2,3)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C7613C8H138N6O62
- Monoisotopic mass : 2230.80984
| P/N | Volume |
| ILGA22S23-600pm | 3x200 pmol |
| ILGA22S23-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary A2F 2x(Sial α-2,3)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C8213C8H148N6O66
- Monoisotopic mass : 2376.86775
| P/N | Volume |
| ILGA2F2S23-600pm | 3x200 pmol |
| ILGA2F2S23-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G2A1(3)(Sial α-2,6)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C6513C8H121N5O54
- Monoisotopic mass : 1936.71443
| P/N | Volume |
| ILGG2A13S26-600pm | 3x200 pmol |
| ILGG2A13S26-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary G2A1(6)F(Sial α-2,6)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C7113C8H131N5O58
- Monoisotopic mass : 2085.77234
| P/N | Volume |
| ILGG2A16FS26-600pm | 3x200 pmol |
| ILGG2A16FS26-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary A2 2x(Sial α-2,6)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C7613C8H138N6O62
- Monoisotopic mass : 2230.80984
| P/N | Volume |
| ILGA22S26-600pm | 3x200 pmol |
| ILGA22S26-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary A2F 2x(Sial α-2,6)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C8213C8H148N6O66
- Monoisotopic mass : 2376.86775
| P/N | Volume |
| ILGA2F2S26-600pm | 3x200 pmol |
| ILGA2F2S26-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary bG0
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C4813C10H97N5O41
- Monoisotopic mass : 1529.59945
| P/N | Volume |
| ILGbG0-600pm | 3x200 pmol |
| ILGbG0-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary bG1(6)
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C5413C10H107N5O46
- Monoisotopic mass : 1691.65227
| P/N | Volume |
| ILGbG16-600pm | 3x200 pmol |
| ILGbG16-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary bG2
- 2 x 13C per GlcNAc
- +8 Da mass gain
- Molecular Formula : C6013C10H117N5O51
- Monoisotopic mass : 1853.70509
| P/N | Volume |
| ILGbG2-600pm | 3x200 pmol |
| ILGbG2-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary bA2 2x(Sial α-2,6)
- 2 x 13C per GlcNAc
- +10 Da mass gain
- Molecular Formula : C8213C10H151N7O67
- Monoisotopic mass : 2435.89593
| P/N | Volume |
| ILGbA22S26-600pm | 3x200 pmol |
| ILGbA22S26-2000pm | 10x200 pmol |
Glycan Standard 13C Biantennary bA2F 2x(Sial α-2,6)
- 2 x 13C per GlcNAc
- +10 Da mass gain
- Molecular Formula : C8813C10H161N7O71
- Monoisotopic mass : 2581.95384
| P/N | Volume |
| ILGbA2F2S26-600pm | 3x200 pmol |
| ILGbA2F2S26-2000pm | 10x200 pmol |
Glycan Standard 13C Triantennary A3G0
- 2 x 13C per GlcNAc
- +10 Da mass gain
- Molecular Formula : C4813C10H97N5O41
- Monoisotopic mass : 1529.59945
| P/N | Volume |
| ILGA3G0-600pm | 3x200 pmol |
| ILGA3G0-2000pm | 10x200 pmol |
Glycan Standard 13C Triantennary A3G0F
- 2 x 13C per GlcNAc
- +10 Da mass gain
- Molecular Formula : C5413C10H107N5O45
- Monoisotopic mass : 1675.65736
| P/N | Volume |
| ILGA3G0F-600pm | 3x200 pmol |
| ILGA3G0F-2000pm | 10x200 pmol |
Glycan Standard 13C Triantennary A3G3F
- 2 x 13C per GlcNAc
- +10 Da mass gain
- Molecular Formula : C7213C10H137N5O60
- Monoisotopic mass : 2161.81583
| P/N | Volume |
| ILGA3G3F-600pm | 3x200 pmol |
| ILGA3G3F-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-G3bcd
- 1 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C7813C6H140N6O61
- Monoisotopic mass : 2214.82387
| P/N | Volume |
| ILGA4-G3bcd-600pm | 3x200 pmol |
| ILGA4-G3bcd-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-LacNAc-G4
- 1 x 13C per GlcNAc
- +7 Da mass gain
- Molecular Formula : C9713C7H173N7O76
- Monoisotopic mass : 2743.01224
| P/N | Volume |
| ILGA4G4LacNAc-600pm | 3x200 pmol |
| ILGA4G4LacNAc-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-G4
- 1 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C8413C6H150N6O66
- Monoisotopic mass : 2376.87669
| P/N | Volume |
| ILGA4G4-600pm | 3x200 pmol |
| ILGA4G4-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-G3abd
- 1 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C7813C6H140N6O61
- Monoisotopic mass : 2214.8236
| P/N | Volume |
| ILGA4G3abd-600pm | 3x200 pmol |
| ILGA4G3abd-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-G1d
- 1 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C6613C6H120N6O51
- Monoisotopic mass : 1890.71822
| P/N | Volume |
| ILGA4G1d-600pm | 3x200 pmol |
| ILGA4G1d-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-G1c
- 1 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C6613C6H120N6O51
- Monoisotopic mass : 1890.71822
| P/N | Volume |
| ILGA4G1c-600pm | 3x200 pmol |
| ILGA4G1c-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-G1a
- 1 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C6613C6H120N6O51
- Monoisotopic mass : 1890.71822
| P/N | Volume |
| ILGA4G1c-600pm | 3x200 pmol |
| ILGA4G1c-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-G0
- 1 x 13C per GlcNAc
- +6 Da mass gain
- Molecular Formula : C6013C6H110N6O46
- Monoisotopic mass : 1728.66540
| P/N | Volume |
| ILGA4G0-600pm | 3x200 pmol |
| ILGA4G0-2000pm | 10x200 pmol |
Glycan Standard 13C Tetraantennary A4-LacNAc-G4F
- 1 x 13C per GlcNAc
- +7 Da mass gain
- Molecular Formula : C10313C7H183N7O80
- Monoisotopic mass : 2889.07015
| P/N | Volume |
| ILGA4G4FLacNAc-600pm | 3x200 pmol |
| ILGA4G4FLacNAc-2000pm | 10x200 pmol |
Glycan Analysis Kits
mAbsolute 키트는 고순도 합성 동위원소 표지 internal standard를 기반으로 MALDI-TOF MS를 이용하여 mAb glycosylation의 절대 정량 분석용 low- and high-throughput glycan 분석 키트입니다. 시료 준비 및 처리 시간이 짧고, internal reference standards와 MALDI profiles을 비교하여 deglycosylated mAb glycans을 정량화 합니다. mAbsolute 키트는 BRUKER Autoflex 및 UltrafleXtreme III MALDI-TOF MS 기반 glycan 절대 정량화에 최적화 되었습니다. 다양한 MALDI-TOF MS 및 기타 질량 분석 기술에 적용하기 위한 검증이 진행 중입니다.
Human IgG N-glycosylation Kit
mAbsolute Human Serum IgG 키트에는 동위원소로 표지된 α-2,6-sialylation을 포함한 pauci-mannose, truncated bi-antennary와 bisected standards가 들어 있습니다. Standards 혼합물은 human serum IgG 1 type 항체의 일반적인 Fc 및 Fab N-glycosylation profile 분석을 위해 설계되었습니다.
| P/N | No. of samples |
| humanK | 10 analysis |
| humanK-HTP | 96 analysis |
mAbs N-glycosylation Kit - Neutral
동위원소로 표지된 Biantennary mAbsolute Neutral kit library는 단일 클론 항체의 전형적인 CHO cell 유래 Fc N-glycosylation profiling을 위해 설계되었습니다.
| P/N | No. of samples |
| mAbNK | 10 analysis |
| mAbNK-HTP | 96 analysis |
