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Overview Ludger Glycoprofiling Services

Ludger's glycoprofiling services are primarily for :
 
· Companies developing new or follow on biopharmaceuticals
· Biopharmaceutical manufacturers
 
These include analytical services for glycan characterization to support IND submissions,
help with setting up ICH-compliant glycoprofiling systems within clients own laboratories
and training in glycoanalysis techniques.
 
Services include :
 
· Determination of non-human sialylation by quantitative determination of NeuAc:
  NeuGc ratio using LudgerTag DMB sialic acid analysis kit and LudgerSep R1
  HPLC with fluorescence detection)
· Monosaccharide composition analysis using either fluorescence HPLC or HPAE-PAD
  (high pH anion exchange with pulse amperometric detection)
· Release of N- and O-linked glycans from the glycoprotein or glycopeptides by :
- a. Endoglycosidases (for N-linked glycans only)
- b. Hydrazinolysis (in N+O mode, N-mode or O-mode)
- c. Ludger LiberateTM glycan release system in N+O mode or O-mode
· Fluorescent labeling of released glycans prior to chromatography or mass
  spectrometry using
·one of the following LudgerTag labeling systems :
- a. LudgerTag 2-AB (2-aminobenzamide) for HPLC and MS
- b. LudgerTag 2-AA (2-aminobenzoic acid) for HPLC, MS and carbohydrate gel
  electrophoresis
- c. LudgerTag AA-Ac for HPLC, MS and capillary electrophoresis
· Oligosaccharide mapping of fluorescently labelled glycans by :
- a. LudgerSep Cx anion exchange HPLC (for glycan charge analysis)
- b. LudgerSep N1 amide HPLC (for analysis of charged and neutral glycans)
- c. Dionex HPAE-FD (fluorescence mode high pH anion exchange used as
  an orthogonal separation to complement the N1 profile)
· Oligosaccharide mapping of non-labelled glycans by HPAE-PAD
· Mass spectrometric analysis (including MSn fragmentation analysis) of derivatized
  and native glycans and glycopeptides by ion trap MS (Shimadzu Axima QIT)
· Glycan sequencing using exoglycosidase digestions in combination with HPLC and
  MS analysis